Plant phenolic compounds as ethidium bromide efflux inhibitors in Mycobacterium smegmatis
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چکیده
منابع مشابه
Thioridazine and chlorpromazine inhibition of ethidium bromide efflux in Mycobacterium avium and Mycobacterium smegmatis.
OBJECTIVES Therapy of AIDS patients infected with Mycobacterium avium is problematic due to its intrinsic resistance to antibiotics. We have characterized the efflux pump activity of M. avium wild-type strain through an automated fluorometric method and correlated it with intrinsic resistance to antibiotics. METHODS M. avium ATCC 25291(T) and Mycobacterium smegmatis mc(2)155 were evaluated fo...
متن کاملDetection of Efflux Pump Using Ethidium Bromide-Agar Cartwheel Method in Acinetobacter baumannii Clinical Isolates
Introduction: In the past decade, multidrug-resistant Acinetobacter baumannii has become one of the most critical challenges in treating infected patients. The AdeABC efflux pump is the most important among the various resistance mechanisms. This pump can force various antibiotics and ethidium bromide out of the bacterial cell to the surrounding environment. Methods: In this study, nine A. baum...
متن کاملFluorometric determination of ethidium bromide efflux kinetics in Escherichia coli
BACKGROUND Efflux pump activity has been associated with multidrug resistance phenotypes in bacteria, compromising the effectiveness of antimicrobial therapy. The development of methods for the early detection and quantification of drug transport across the bacterial cell wall is a tool essential to understand and overcome this type of drug resistance mechanism. This approach was developed to s...
متن کاملEfflux-mediated response of Staphylococcus aureus exposed to ethidium bromide.
OBJECTIVES By adapting an antibiotic-susceptible Staphylococcus aureus strain to increasing concentrations of ethidium bromide, a known substrate of efflux pumps (EPs), and by phenotypically and genotypically analysing the resulting progeny, we characterized the molecular mechanisms of S. aureus adaptation to ethidium bromide. METHODS S. aureus ATCC 25923 was grown in increasing concentration...
متن کاملFarnesol, a potential efflux pump inhibitor in Mycobacterium smegmatis.
The active multidrug efflux pump (EP) has been described as one of the mechanisms involved in the natural drug resistance of bacteria, such as mycobacteria. As a result, the development of efflux pumps inhibitors (EPIs) is an important topic. In this study, a checkerboard synergy assay indicated that farnesol both decreased the minimum inhibitory concentration (MIC) of ethidium bromide (EtBr) 8...
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ژورنال
عنوان ژورنال: Journal of Antimicrobial Chemotherapy
سال: 2008
ISSN: 0305-7453,1460-2091
DOI: 10.1093/jac/dkn178